XIV International Scientific Agriculture Symposium (AGROSYM), Sarajevo, Bosna-Hersek, 5 - 08 Ekim 2023, ss.617
This study aims to isolate bacteria with probiotic properties from canine natural milk microbiota and to investigate their potentials to be used as a food supplement for animal health. In this context, bacteria were isolated from the milk microbiota of the Kangal Shepherd Dog known as a traditional Turkish breed of large livestock guardian dog. Isolate exhibited a broad anti-microbial activity against pathogens was selected and identified as Limosilactobacillus reuteri using 16S rRNA sequence analysis. Anti-biofilm activity was performed using crystal violet staining and 95% inhibition on biofilm formation of E. coli O157 was detected. The strain was evaluated within the scope of EFSA safety criteria, and found as non-hemolytic and resistant to gentamicin, kanamycin, and streptomycin antibiotics. Under the simulated gastrointestinal system conditions, the strain displayed resistance to low pH, pepsin, pancreatin, and bile salt. Viable cells of the isolate did not exhibit toxicity on Caco-2 colon epithelial cell line. The adhesion capability of viable cells on Caco-2 cells decreased the adhesion rate of E. coli (91.76%). When the Caco-2 cells were stimulated with LPS and then incubated with live cells of L. reuteri, pro-inflammatory cytokines were decreased while the anti-inflammatory cytokines were increased. The enzymatic activities of the strain were also determined using API kits. In conclusion, it was determined that L. reuteri isolated from canine natural milk microbiota with its probiotic properties can be used as a safe and effective strain in food supplements for pet animals.