Akademik Veri Yönetim Sistemi
Journal of Animal and Veterinary Advances, cilt.12, sa.3, ss.382-386, 2013 (SCI-Expanded)
Two primary flight muscles of Miniopterus schreibersi were studied using morphological and histochemical analysis. All animals were killed with an overdose of sodium pentabarbital administered intraperitoneally. Muscles were dissected free, cleaned of excess fasia, blotted dry and weighed. Their proximal and distal portions were mounted in gum tragacanth on cork, quick-frozen by immersionin isopentane cooled to about -160°C. Transverse serial sections (10-12 μm thickness) were obtained with a freezing ciyostat sections from each muscle were stained with Nicotinamide Adenine Dinucleotide Tetrazolium Reductase (NADH-TR) to assess oxidative capacity. The method was used demonstate Succinate Dehydrogenase (SDH) activity. Sections of each muscle were stained using myosin Adenosine Triphosphatase (mATPase). Two fast twitch fiber types are histochemically identified in pectoralis muscles of M schreibersi. These were classified as type Ha and b according to glycine-calcium-formalin preincubation staining protocol for myosin ATPase. The primary flight muscles, serratus ventalis included type I, Ha and b fibers. Type I fibers in serratus ventralis were highly oxidative as stained darkly for NADH-TR. All type Ha fibers exhibited relatively intense staining properties for NADH-TR and SDH suggesting a intermediate oxidative capacity. InM. schreibersi primary flight muscles, type lib fibers were low oxidative as indicated by light reaction for NADH-TR. Fiber ratios of M.schreibersi for the pectoral muscle are fiber type Ha 87% and fiber type lib 13%. Fiber ratios for serratus ventralis are fiber type I 14%, fiber type Ha 70%) and fiber type lib 16%. © Medwell Journals, 2013.