A phage DNA injection-blocking type resistance mechanism encoded by chromosomal DNA in Lactococcus lactis subsp. lactis PLM-18

Akcelik M.

MILCHWISSENSCHAFT-MILK SCIENCE INTERNATIONAL, vol.53, no.11, pp.619-622, 1998 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 53 Issue: 11
  • Publication Date: 1998
  • Journal Name: MILCHWISSENSCHAFT-MILK SCIENCE INTERNATIONAL
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.619-622
  • Ankara University Affiliated: No

Abstract

Lactococcus lactis subsp. lactis strain PLM-18 has a phage resistance activity against empty set la2 which resulted in phage DNA injection-blocking. After adsorption of empty set la2 to PLM-18 and its plasmid free derivative MA-12 cells with the same adsorption rate (98.4%), phage DNA was detected in neither PLM-18 nor MA-12 cells while detecting ar la2 DNA in sensitive host LLA-111 after 5 min following phage adsorption. However, electrotransformation of phage DNA into resistant hosts, PLM-18 and MA-12, resulted in release of phage progeny on the contrary of conventional infection assays.