Intragenic haplotypes at the bovine CSN1S1 locus

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Jann O., Prinzenberg E., Brandt H., Williams J., Ajmone-Marsan P., Zaragoza P., ...More

ARCHIV FUR TIERZUCHT-ARCHIVES OF ANIMAL BREEDING, vol.45, no.1, pp.13-21, 2002 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 45 Issue: 1
  • Publication Date: 2002
  • Doi Number: 10.5194/aab-45-13-2002
  • Journal Name: ARCHIV FUR TIERZUCHT-ARCHIVES OF ANIMAL BREEDING
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.13-21
  • Keywords: casein, haplotype, SSCP, cattle, POLYMERASE CHAIN-REACTION, MILK PROTEIN POLYMORPHISMS, ALPHA-S1-CASEIN ALLELE, PRODUCTION TRAITS, CATTLE, DNA, VARIANTS, GENE, FREQUENCIES, BREEDS
  • Ankara University Affiliated: Yes

Abstract

A new alternative genotyping method based on PCR-SSCP was developed for direct differentiation of the CSN1S1 alleles B and C in the coding region. In addition a PCR-RFLP test based on a MaeIII restriction site in the promoter region of CSN1S1, reported in the literature as an alternative test for the differentiation of CSN1S1*B and C was used and the alleles named b and c. Genotyping of 649 animals belonging to 17 European and Turkish cattle breeds showed differences in occurrence and frequency of the alleles. CSN1S1*B occurred in all breeds with frequencies varying from 0.50 in Anatolian Blackup to 1.0 in e.g. Ayrshire. CSN1S1*b on the other hand varied from 0.63 in Jersey, 0.97 in Ayrshire to 1.0 in e.g. Angler.