Akademik Veri Yönetim Sistemi
9th International Congress on Advances in Veterinary Sciences & Technics (ICABB) Aksaray-Hearth of Cappadocia-, TÜRKIYE on September 23-27, 2024 , Aksaray, Türkiye, 23 - 27 Eylül 2024, ss.36-46
Bovine adenoviruses (BAdV) are a group of viruses associated with various diseases affecting cattle, including respiratory and enteric infections, and some of them have oncogenic potential. BAdV are classified into two genera: Mastadenovirus (BAdV-1, 2, -3, -9 and -10) and Atadenovirus (BAdV-4, -5, -6, -7, and -8). BAdV-1 and -3 are recognized as important respiratory pathogens in cattle, leading to acute or subacute viral diseases characterized by symptoms such as fever, naso-ocular discharge, and pneumonia. In this study, a total of 24 swab samples from calves aged 0 to 6 months with respiratory symptoms were tested for the presence of bovine mastadenoviruses. After the viral DNA extraction, samples were subjected to PCR using primers targeting the hexon gene region of mastadenoviruses. As a result of PCR, six samples demonstrated the anticipated amplicon size (with a 25% positivity rate). Subsequently, five samples underwent sequencing, and phylogenetic analyses were conducted. Phylogenetic analysis revealed that all sequenced samples were grouped within the BAdV-1 serotype. Additionally, BAdV-1 was identified as the sole causative agent in five of the positive samples. Although investigations have shown the existence of BAdVs in Türkiye, there is limited data on the molecular characterization of the bovine adenoviruses in our country. This study, which was conducted on samples obtained from calves with respiratory system disease in Kastamonu, is the first report on the molecular characterization of BAdV-1 in Türkiye. In conclusion, this investigation has shown that BAdVs have played as an important agent in respiratory system infections. Further studies on the molecular characterization and epidemiology of these viruses would contribute to a better understanding of the role of bovine adenoviruses in respiratory disease.