Multivariate Optimization of Separation Conditions for Simultaneous Determination of Acemetacin and Chlorzoxazone in a Pharmaceutical Preparation by HPLC Using Response Surface Methodology

Aybaba C., PALABIYIK İ. M., ÇAĞLAYAN M. G., Onur F.

JOURNAL OF AOAC INTERNATIONAL, cilt.96, sa.4, ss.723-729, 2013 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 96 Sayı: 4
  • Basım Tarihi: 2013
  • Doi Numarası: 10.5740/jaoacint.11-092
  • Dergi Adı: JOURNAL OF AOAC INTERNATIONAL
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.723-729
  • Ankara Üniversitesi Adresli: Evet

Özet

A new, fast, accurate, precise, and sensitive RP-HPLC method for the simultaneous determination of acemetacin and chlorzoxazone has been developed. Response surface methodology with a central composite design was used to optimize the acetonitrile and ammonium acetate percentage in the mobile phase and pH of ammonium acetate. The optimum separation was achieved on a C18 column (250 x 4.6 mm id, 5 mu m particle size) using the mobile phase methanol-acetonitrile-0.02 M ammonium acetate, pH 9.4 (25 + 35 + 40, v/v/v) at a flow rate of 1.5 mL/min; UV detection at 270 nm, and cyanocobalamin as an internal standard. This developed method was validated and successfully applied to a coated tablet pharmaceutical preparation.