Inhibitory effects of indole alpha-lipoic acid derivatives on nitric oxide production in LPS/IFN gamma activated RAW 264.7 macrophages

KARABAY A. Z., KOÇ A., Gurkan-Alp A. S., Buyukbingol Z., Buyukbingol E.

CELL BIOCHEMISTRY AND FUNCTION, cilt.33, sa.3, ss.121-127, 2015 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 33 Sayı: 3
  • Basım Tarihi: 2015
  • Doi Numarası: 10.1002/cbf.3095
  • Dergi Adı: CELL BIOCHEMISTRY AND FUNCTION
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.121-127
  • Anahtar Kelimeler: indole, alpha-lipoic acid, RAW 264, 7, iNOS, inflammation, DIHYDROLIPOIC ACID, ANTIOXIDANTS
  • Ankara Üniversitesi Adresli: Evet

Özet

Alpha-lipoic acid (-lipoic acid) is a potent antioxidant compound that has been shown to possess anti-inflammatory effects. RAW 264.7 macrophages produce various inflammatory mediators such as nitric oxide, IL-1, IL-6 and TNF-alpha upon activation with LPS ( Lipopolysaccharide) and IFN (interferon gamma). In this study, the effect of 12 synthetic indole -lipoic acid derivatives on nitric oxide production and iNOS (inducible nitric oxide synthase) protein expression in LPS/IFN activated RAW 264.7 macrophages was determined. Cell proliferation, nitric oxide levels and iNOS protein expression were examined with thiazolyl blue tetrazolium blue test, griess assay and western blot, respectively. Our results showed that all of the indole -lipoic acid derivatives showed significant inhibitory effects on nitric oxide production and iNOS protein levels (p<0.05). The most active compounds were identified as compound I-4b, I-4e and II-3b. In conclusion, these indole -lipoic acid derivatives may have the potential for treatment of inflammatory conditions related with high nitric oxide production. Copyright (c) 2015 John Wiley & Sons, Ltd.