Early-Onset Preeclampsia-Derived hUC-MSCs Exhibit Accelerated Initial Culture Kinetics Without Sustained Growth Advantage: A Comparative In Vitro Study
Stem Cells International, cilt.2026, sa.1, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 2026 Sayı: 1
- Basım Tarihi: 2026
- Doi Numarası: 10.1155/sci/3980640
- Dergi Adı: Stem Cells International
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, EMBASE, Directory of Open Access Journals, Academic Search Ultimate (EBSCO), Biomedical Reference Collection: Corporate Edition (EBSCO), Health Research Premium Collection (ProQuest), Pharma Collection (ProQuest)
- Anahtar Kelimeler: cell culture, early-onset preeclampsia (EOPE), human umbilical cord-derived mesenchymal stromal cells (hUC-MSCs), late-onset preeclampsia (LOPE), population doubling time
- Ankara Üniversitesi Adresli: Evet
Özet
Preeclampsia (PE) is a pregnancy-related disorder associated with significant maternal and fetal morbidity, and intrauterine conditions may influence the biological behavior of human umbilical cord (UC)-derived mesenchymal stem cells (hUC-MSCs). However, comparative data between early-onset PE (EOPE) and late-onset PE (LOPE) remain limited. This study aimed to compare the in vitro growth kinetics of hUC-MSCs derived from pregnancies complicated by EOPE and LOPE. In this comparative in vitro study, UC samples (n = 66) were classified into three groups according to the maternal clinical condition: control (n = 22), EOPE (n = 22), and LOPE (n = 22). Cells were isolated using a mechanical explant method and cultured under standard conditions. Culture parameters, including cell-outgrowth time, time to 90% confluency (P0), first passage duration (P1), and total culture duration (P0+P1), were recorded. Population doubling time (PDT) was calculated using both outgrowth-adjusted and P1-based approaches, and phenotypic characterization was performed by flow cytometry. Cell-outgrowth time did not differ significantly among the groups (p = 0.930). P0 duration differed significantly among the groups (p < 0.001). EOPE-derived cells exhibited a shorter P0 duration (19.00 ± 2.19 days) than both control-derived cells (21.65 ± 1.63 days; Tukey-adjusted p = 0.0004) and LOPE-derived cells (20.71 ± 2.35 days; Tukey-adjusted p = 0.0266). P1 duration and total culture duration did not differ significantly among the groups (p = 1.000 and p = 0.090, respectively). The outgrowth-adjusted group-level PDT estimate was lower in EOPE (16.6 h) than in LOPE (18.4 h) and controls (19.1 h), whereas the P1-based PDT estimates were closely similar across the groups (~32 h). Total cell yield and viability were numerically similar across the groups. These findings indicate that EOPE-derived hUC-MSCs exhibit an early culture-specific kinetic difference, predominantly reflected by a shorter P0 duration, without a sustained difference in subsequent P1 expansion or total culture duration. The accelerated early growth may reflect adaptive responses to the intrauterine environment rather than enhanced functional potential, suggesting that early culture parameters may serve as sensitive indicators of disease-related alterations.