Detection and characterisation of sheep-associated malignant catarrhal fever infection from ruminants by using tegument and gB gene sequences of OvHV-2

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Oğuzoğlu T. Ç., Salar S., Adiguzel E., Demirden C., Ulgenalp O.

ONDERSTEPOORT JOURNAL OF VETERINARY RESEARCH, vol.87, no.1, 2020 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 87 Issue: 1
  • Publication Date: 2020
  • Doi Number: 10.4102/ojvr.v87i1.1886
  • Journal Name: ONDERSTEPOORT JOURNAL OF VETERINARY RESEARCH
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Agricultural & Environmental Science Database, BIOSIS, CAB Abstracts, EMBASE, MEDLINE, Veterinary Science Database, Directory of Open Access Journals
  • Keywords: malignant catarrhal fever, tegument gene, gB gene, OvHV-2, Turkey, CATTLE, VIRUS, IMMUNIZATION, AGENT
  • Ankara University Affiliated: Yes

Abstract

In this study, positive blood and organ samples were obtained from different mixed herds of sheep and cattle against ovine herpesvirus 2 (OvHV-2) infection. Target-positive DNA was sequenced and compared with worldwide distributed OvHV-2 sequences. Tegument gene (422 base pairs) and glycoprotein B (gB) gene (2800 base pairs) amplicons of OvHV-2 genome were used for understanding of epidemiology of malignant catarrhal fever (MCF) infection in Turkey. The results of nucleotide sequencing of polymerase chain reaction (PCR) products indicated presence of sheep-associated form for MCF infection in Turkey. Although the obtained sequences were genetically different from each other, it was found that genetic variations were limited.