Akademik Veri Yönetim Sistemi
15th NATIONAL – 1st INTERNATIONAL CONGRESS OF HISTOLOGY AND EMBRYOLOGY, 26 - 28 Mayıs 2022, ss.141
EFFECT OF SHORT ABSTINENCE PERIOD ON SEMEN PARAMETERS OF NORMOZOOSPERMIC HEAVY SMOKER MEN: A PROSPECTIVE COMPARATIVE STUDY 1Bahar Şahin Cansoy | 2Özlem Doğan | 1Ekin Baysal | 1Sevim Aydın | 1Elmas Yaren Suiçmez 1 İskender Sinan Özkavukcu 1Ankara University Faculty of Medicine Department of Histology and Embryology, 2Ankara University Faculty of Medicine Department of Biochemistry, baharsahin41@hotmail.com Introduction: In the process from spermatogenesis and spermiogenesis to ejaculation, testis, epididymis, vas deferens and accessory reproductive glands function. Toxic agents to which spermatozoa will be exposed at these stages can affect the quality of spermatozoa and adversely affect parameters such as fertilization, embryo quality, pregnancy and the health status of the born offspring. This interaction occurs most strikingly during the storage process of spermatozoa in the epididymis. Cigarette consumption, which is in the first place in the etiology of mortality and morbidity, causes excessive production of various toxic agents, especially reactive oxygen radicals (ROR) in the body. At the same time, the effect of cigarette consumption on the success rates of assisted reproductive techniques (ART) has been extensively studied. In this study, the possible positive effects of short absinence time on semen parameters, sperm chromatin integrity and sperm DNA fragmentation by reducing the ROR exposure of spermatozoa in the epididymis in men with heavy smoking were investigated by comparing them with control groups. Materials and methods: After 7 and 1 days of sexual abstinence, semen samples were collected by masturbation from heavy smokers (≥20) and non-smoker normozoospermic volunteers. Following liquefaction, spermatozoon concentration (with Neubauer hemocytometry), motility (with computer-assisted sperm analysis), morphology (with Diff-Quick stain), persistent histone (with aniline blue stain) and viability (with eosin nigrosine stain) rates were evaluated. DNA fragmentation (TUNEL labeling) rates and intracellular ROR levels (DCFDA staining) were determined by the flow cytometry method. In addition, malondialdehyde (MDA) levels in seminal plasma obtained after centrifugation were measured spectrophotometrically. Results: In all groups, semen volume and total sperm count increased significantly in direct proportion to the duration of sexual abstinence. No significant difference was observed between concentration, motility, morphology, persisted histone, viability, DNA fragmentation, and intracellular ROR levels in smokers and non smokers. It was determined that the duration of sexual abstinence did not cause a significant change in these parameters. After long periods of sexual abstinence, seminal plasma MDA levels were found to be significantly higher in the non-smoker group. Conclusion: The World Health Organization recommends sexual abstinence for 2 to 7 days for men who apply for semen analysis. In the light of the findings obtained in our study, it was concluded that these recommended times were optimal and that despite heavy cigarette consumption, semen parameters, DNA fragmentation rates and chromatin packaging levels were not affected. Although our findings are in line with similar studies in the literature, studies that evaluate clinical data and include patients with abnormal semen parameters are needed. As a result, it was evaluated that there is no need to recommend a short period of sexual abstinence in normozoospermic cases of heavy smokers who applied to ART clinics.