The combined effects of 5-fluorouracil and Turkish propolis extract on EPGF and KRAS expressions and apoptotic cascade changing in rat colon cancer model

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KURTDEDE E., Alçiğir M. E., Şahin Y.

Ankara Universitesi Veteriner Fakultesi Dergisi, cilt.72, sa.2, ss.219-230, 2025 (SCI-Expanded, Scopus, TRDizin)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 72 Sayı: 2
  • Basım Tarihi: 2025
  • Doi Numarası: 10.33988/auvfd.1513024
  • Dergi Adı: Ankara Universitesi Veteriner Fakultesi Dergisi
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, CAB Abstracts, EMBASE, Veterinary Science Database, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.219-230
  • Anahtar Kelimeler: 5-Flourouracil, Azoxymethane, Cancer, Immunohistochemistry, Propolis, Rat colon
  • Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
  • Ankara Üniversitesi Adresli: Evet

Özet

This study aimed to investigate the effectiveness of 5-fluorouracil (5-FU) and Turkish propolis extract on the colon cancer model induced by azoxymethane (AOM) in Wistar rats. Study groups were designed as cancer group (AOM), control group, AOM+5-FU group, AOM+propolis group, AOM+Propolis+5-FU group. The findings showed that there was a significant decrease in WBC, and lymphocyte levels in the treatment groups AOM+5FU and AOM+Propolis+5FU compared to the AOM group (P˂0.0001, P˂0.005, respectively). The increase in TAS level and TOS level were found to be significant in the AOM+Propolis+5FU group compared to the AOM group (P˂0.0001). Compared to the the AOM group, caspase-3, caspase-8 and bax levels were significantly increased and Bcl-2 evel was significantly lower in the 5 AOM+Propolis+5FU group (P˂0.0001, P˂0.05, P˂0.0001, P˂0.0001, respectively). In the 5-FU and propolis used-therapy groups, decreased anaplasia in colonic cells. In conclusion, we strongly believe that as a chemotherapeutic, 5-FU, and propolis might have a stopper effect on cellular anaplasia over extrinsic apoptotical pathway. We also believe that this antiproliferative effect on cellular anaplasia can appear if the combined therapy is preferred.