In situ reactive extraction of lactic acid from fermentation media

Tik N., Bayraktar E., Mehmetoglu U.

Journal of Chemical Technology and Biotechnology, cilt.76, sa.7, ss.764-768, 2001 (SCI-Expanded, Scopus) identifier identifier

  • Yayın Türü: Makale / Özet
  • Cilt numarası: 76 Sayı: 7
  • Basım Tarihi: 2001
  • Doi Numarası: 10.1002/jctb.449
  • Dergi Adı: Journal of Chemical Technology and Biotechnology
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.764-768
  • Anahtar Kelimeler: reactive extraction, lactic acid production, immobilised Lactobacillus delbrueckii, AMINE EXTRACTION, CITRIC-ACID, SOLVENT
  • Ankara Üniversitesi Adresli: Evet

Özet

Extractive lactic acid fermentation was investigated in the presence of sunflower oil and Alamine-336 (with oleyl alcohol as the diluent solvent). Lactic acid was produced in various media at 37°C using Lactobacillus delbrueckii (NRRL-B 445). First, the effects of oleyl alcohol (33.3%, v/v), immobilisation, and immobilisation in the presence of sunflower oil (5, 10, 15%, v/v) on lactic acid production were investigated. It was found that oleyl alcohol did not affect production while addition of sunflower oil increased lactic acid production from 10.22 to 16.46 gdm-3. On the other hand, a toxic effect was observed for oleyl alcohol solutions containing 15-50% (v/v) Alamine-336. A maximum total lactic acid concentration of 25.59 gdm-3 was obtained when an oleyl alcohol solution containing 15% (v/v) Alamine together with immobilised cells with 15% (v/v) sunflower oil was used. This value was about 2.5 times that obtained from fermentation without organic solutions. © 2001 Society of Chemical Industry.