Urea biosensors based on PVC membrane containing palmitic acid

Karakus E., Pekyardimci S., Kilic E.

ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY, vol.33, no.3, pp.329-341, 2005 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 33 Issue: 3
  • Publication Date: 2005
  • Doi Number: 10.1081/bio-200066632
  • Journal Name: ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.329-341
  • Keywords: urea, polyvinylchloride, carboxylated polyvinylchloride, palmitic acid, nonactin, POTENTIOMETRIC BIOSENSOR, ENZYME ELECTRODE, SENSOR, BLOOD, SERUM
  • Ankara University Affiliated: No

Abstract

A new urea biosensor was prepared by immobilizing urease with four different procedures on poly(vinylchloride) (PVC) ammonium membrane electrode containing palmitic acid by using nonactine as an ammonium-ionophore. The analytical characteristics were investigated and were compared those of the biosensor prepared by using carboxylated PVC. The effect of pH, buffer concentration, temperature, urease concentration, stirring rate and enzyme immobilization procedures on the response to urea of the enzyme electrode were investigated. The linear working range and sensitivity of the biosensor were also determined. The urea biosensor prepared by using the PVC membranes containing palmitic acid showed more effective performance than those of the carboxylated PVC based biosensors. Additionally, urea assay in serum was successfully carried out by using the standard addition method.