The Effects of MBL1 Gene Polymorphism on Subclinical Mastitis in Holstein Cows

AKSEL E. G., AKÇAY A., ARSLAN K., Sohel M. H., GÜNGÖR G., AKYÜZ B.

KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI, cilt.27, sa.3, ss.389-395, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 27 Sayı: 3
  • Basım Tarihi: 2021
  • Doi Numarası: 10.9775/kvfd.2021.25714
  • Dergi Adı: KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, CAB Abstracts, EMBASE, Veterinary Science Database, Directory of Open Access Journals, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.389-395
  • Anahtar Kelimeler: Cow, MBL1, Multiple logistic regression analysis, SNP, Subclinical mastitis, DAIRY-COWS, BINDING, PREVALENCE, RESISTANCE, PATHOGENS
  • Ankara Üniversitesi Adresli: Evet

Özet

This study aims to investigate the effects of three single nucleotide (SNPs) polymorphisms of the MBL1 gene on subclinical mastitis for Holstein cows. For this study, a total of 151 Holstein cows were selected in their third lactation. The subclinical mastitis conditions were determined using the California Mastitis Test (CMT). Genotyping was carried out with the PCR-RFLP method. The results revealed that the wild-type allele frequencies were 0.72 (G allele), 0.40 (G allele), and 0.37 (T allele) for 1252 G>A, 2534 G>A, and 2569 T>C SNPs, respectively. Among the investigated SNPs, only the 1252 G>A SNP was not found in Hardy-Weinberg equilibrium. The effects of SNPs on subclinical mastitis were modeled using multiple logistic regression analysis. The established model can identify cows with subclinical mastitis with a separation efficiency of 62.3%. It was thought that 1252 G>A and 2534 G>A SNPs may affect subclinical mastitis rates.