The Effects of MBL1 Gene Polymorphism on Subclinical Mastitis in Holstein Cows

AKSEL E. G., AKÇAY A., ARSLAN K., Sohel M. H., GÜNGÖR G., AKYÜZ B.

KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI, vol.27, no.3, pp.389-395, 2021 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 27 Issue: 3
  • Publication Date: 2021
  • Doi Number: 10.9775/kvfd.2021.25714
  • Journal Name: KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, CAB Abstracts, EMBASE, Veterinary Science Database, Directory of Open Access Journals, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.389-395
  • Keywords: Cow, MBL1, Multiple logistic regression analysis, SNP, Subclinical mastitis, DAIRY-COWS, BINDING, PREVALENCE, RESISTANCE, PATHOGENS
  • Ankara University Affiliated: Yes

Abstract

This study aims to investigate the effects of three single nucleotide (SNPs) polymorphisms of the MBL1 gene on subclinical mastitis for Holstein cows. For this study, a total of 151 Holstein cows were selected in their third lactation. The subclinical mastitis conditions were determined using the California Mastitis Test (CMT). Genotyping was carried out with the PCR-RFLP method. The results revealed that the wild-type allele frequencies were 0.72 (G allele), 0.40 (G allele), and 0.37 (T allele) for 1252 G>A, 2534 G>A, and 2569 T>C SNPs, respectively. Among the investigated SNPs, only the 1252 G>A SNP was not found in Hardy-Weinberg equilibrium. The effects of SNPs on subclinical mastitis were modeled using multiple logistic regression analysis. The established model can identify cows with subclinical mastitis with a separation efficiency of 62.3%. It was thought that 1252 G>A and 2534 G>A SNPs may affect subclinical mastitis rates.