Akademik Veri Yönetim Sistemi
Bratislava Medical Journal, cilt.126, sa.11, ss.2929-2944, 2025 (SCI-Expanded)
Aim: The objective of our study was to investigate the apoptotic effect of ferulic acid (FA) on endometrial cancer (EC) cells through the application and analysis of a nanoparticle known as Zeolitic Imidazolate Frameworks-8 (ZIF-8). Methods: FA molecules were coated with ZIF-8 (FA-ZIF-8) and subjected to analysis using several techniques including Scanning Electron Microscopy (SEM), Surface Enhanced Raman Spectroscopy, Fourier Transform Infrared Spectroscopy (FT-IR), UV-visible spectroscopy and elemental mapping analysis, and energy dispersive X-ray spectroscopy (EDS). XTT kit, quantitative real-time PCR, immunocytochemistry, TUNEL, wound healing assay and colonization test was used to determine the apoptotic effects of FA-ZIF-8 on Ishikawa and HEC1B Cells. Results: SEM showed that the particles were hexagonal with an average diameter of 250 nm, SERS mapping showed that the particles were distributed in the middle and on the sides, FT-IR spectrum showed the presence of main functional groups and UV spectrum showed maximum absorbance at 215 nm. IC50 dose of FA-ZIF-8 for ishikawa cell was 300,03 μg/mL at 72 h, and for HEC1B cell was 467,56 μg/mL at 72 h. Caspase-3 and Bax/Bcl-2 gene ratios (2.03 fold in Ishikawa, and 3.53 fold in HEC1B) and apoptotic index (AI) increased (p<0.05), cell proliferation and migration decreased in FA-ZIF-8 groups. Conclusions: This study showed that ZIF-8 nanoparticles encapsulating FA could be a therapeutically effective treatment for the EC cells.