Akademik Veri Yönetim Sistemi
PAKISTAN JOURNAL OF AGRICULTURAL SCIENCES, cilt.54, sa.2, ss.255-261, 2017 (SCI-Expanded)
The study presents the in vitro potential of preconditioned plumular apices explants of peanut for shoot regeneration. Mature embryo explants were isolated and preconditioned for 10 days on MS medium with 10 and 20 mg/l BAP (6-Benzylaminopurine) followed by postconditioning of isolated plumular apices postconditioned on MS medium enriched with 0.25-2.0 mg/l BAP singly or with 0.25 mg/l NAA (1-Naphthaleneacetic acid). Preconditioning of explants using 20 mg/l BAP was found to be more regenerative to induce 4.38 shoots with shorter shoots (0.75 cm) compared to 3.50 shoots and shoot length of 1.25 cm when preconditioned with 20 mg/l BAP. Number of shoots per explants ranged 2.30-5.36 and 3.17-6.0 respectively on explants preconditioned with 10 and 20 mg/l BAP and postconditioned on 0.25-2.0 mg/l BAP -0, 0.25 mg/l NAA with shoot length in range of 1.01-2.30 cm and 0.74-1.80 cm, respectively. Maximum number of shoots (6.0) with stunted shoots (0.74 cm) were recorded from 20 mg/l preconditioning followed by culture on medium containing provided with 1.0 mg/l BAP+0.25 mg/l NAA. Irrespective of preconditioning treatments, minimum number of shoots per explants but relatively longer shoots were scored on MS medium provided with 0.25 mg/l BAP+0.25 mg/l NAA. Regenerated shoots were successfully rooted using 0.25-1.0 mg/l IBA. They were transferred to pots containing peat moss that resulted in successful acclimatization of in vitro regenerated plantlets at room temperature.