Identification of gene expression profiles in Leishmania major infection by integrated bioinformatics analyses

Ulusan Ö., Mert U., Sadiqova A., Ozturk S., Caner A.

ACTA TROPICA, cilt.208, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 208
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1016/j.actatropica.2020.105517
  • Dergi Adı: ACTA TROPICA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Communication Abstracts, EMBASE, Geobase, MEDLINE, Metadex, Veterinary Science Database, Civil Engineering Abstracts
  • Anahtar Kelimeler: Leishmania major (L. major), Macrophages, BALB/c mice, Gene expression, TREM signaling, CUTTING EDGE, TREM-1, INDUCTION, MICE, NEUTROPHILS, ACTIVATION, REVEALS, SKIN
  • Ankara Üniversitesi Adresli: Hayır

Özet

Gene expression profiling in mouse models of leishmaniasis has given useful information to understand the molecular pathways active in lesions and to discover new diagnostic/therapeutic targets. Although the host response plays a critical role in protection from leishmaniasis and promoting disease severity, there are still unexplained aspects in the mechanism of non-healing cutaneous lesions, which need biomarkers for both targeted- therapy and diagnosis. To address this, transcriptional profiling of the skin lesions obtained from BALB/c mice infected with Leishmania major and healthy skin from naive mice were evaluated by bioinformatics analysis, and then the results were validated by Revers Transcriptase-PCR. Five genes among the up-regulated differentially expressed genes named FCGR4, CCL4, CXCL9, Arg1 and IL-1 beta were found to have relatively high diagnostic value for CL due to L. major. Pathway analysis revealed that Triggering Receptor Expressed On Myeloid Cells 1 (TREM1) signaling pathways are active in cutaneous lesions, providing new insights for the understanding and treatment of leishmaniasis.