In vitro and in vivo activity of Melittin combined with imipenem and meropenem against carbapenem-resistant A. baumannii

Genç Karadenİz D., KAŞKATEPE B., Öztürk Ş., Güvenİr M.

Future Microbiology, 2025 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Basım Tarihi: 2025
  • Doi Numarası: 10.1080/17460913.2025.2572931
  • Dergi Adı: Future Microbiology
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, CAB Abstracts, Chemical Abstracts Core, EMBASE, MEDLINE, Veterinary Science Database
  • Anahtar Kelimeler: Acinetobacter baumannii, antimicrobial resistance, Galleria mellonella, Melittin, time kill assay
  • Ankara Üniversitesi Adresli: Evet

Özet

Objectives: To investigate the antimicrobial efficacy of Melittin (MEL) against carbapenem-resistant Acinetobacter baumannii isolates as a potential alternative treatment option. Materials and methods: Minimum Inhibitory Concentrations (MICs) of MEL, imipenem (IMP), and meropenem (MER) were evaluated through the microdilution method in 10 resistant isolates. Synergistic interactions were assessed using checkerboard, time-kill assays, and an in vivo Galleria mellonella model. Results: MEL MICs ranged from 31.25–62.5 mg/L. Only the M5 isolate showed synergy with both combinations. Fractional Inhibitory Concentration Index (FICI) values were 0.375 for MEL+MER and 0.5 for MEL+IMP. Larvae treated with MEL combinations had increased survival rates compared to the infection group. Conclusions: MEL appears to be a promising therapeutic candidate against carbapenem-resistant A. baumannii, and its combination with carbapenems may enhance in vivo efficacy.