Akademik Veri Yönetim Sistemi
2024 International Collaborative Conference of Modern Agricultural Technologies, ICCMAT 2024, Anbar, Ramadi, Irak, 9 - 10 Ekim 2024, cilt.1449, (Tam Metin Bildiri)
Medicinal plants represent a reservoir of economically significant compounds with therapeutic potential. The vast chemical properties and diversity essential in these plants has gathered considerable interest from researchers for drug discovery for human welfare. With this aspect, Hypericum species exhibit notable pharmacological secondary biochemicals like hypericin and hyperforin. But, the direct extraction of these valuable compounds from field-grown Hypericum plants shows challenges, including concerns regarding the quality and quantity of the extracted compounds that requiring uniformity in plant growth. Therefore, this research aims on establishment of a robust report for regeneration via callus induction (callogenesis) in the medically important Hypericum perforatum L. In vitro grown seedlings were source of explants, comprising leaf, apical meristem, and cotyledon segments. Results revealed 100% callogenesis induced for both leaf and apical meristem on MS medium supplemented with 1 mg L-1 BAP and 0.5 mg L-1 NAA. On the other hand, cotyledon segments exhibited maximum callus induction (100%) with 4 mg L-1 BAP and 2 mg L-1 NAA. The maximum number of shoots per explant (19.5) was achieved with leaf explants on MS basal media contains 1 mg L-1 BAP and 0.5 mg L-1 NAA. The in vitro plantlets with healthy roots were acclimatized in a pot filled with peat moss and perlite (1:1 ratio), and subsequently transplanted to fields for hardening. This research offers reliable regeneration protocol for the micropropagation of a multitude of plants that facilitating commercial and economic yield of plant secondary biochemicals such as hypericin and hyperforin, while mitigating harm to the natural resources of H. perforatum L.