A chemometric optimization of method for determination of nitrosamines in gastric juices by GC-MS

AKYÜZ M., ATA Ş., DİNÇ E.

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, vol.117, pp.26-36, 2016 (SCI-Expanded, Scopus) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 117
  • Publication Date: 2016
  • Doi Number: 10.1016/j.jpba.2015.08.021
  • Journal Name: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.26-36
  • Keywords: Nitrosamines, Chemometric, GC-MS, Gastric juices, Diseases, VOLATILE N-NITROSAMINES, CHROMATOGRAPHY-MASS-SPECTROMETRY, PRESSURE CHEMICAL-IONIZATION, LIQUID-CHROMATOGRAPHY, GAS-CHROMATOGRAPHY, NITROSO COMPOUNDS, CANCER, EXPOSURE, LIVER, FOOD
  • Ankara University Affiliated: Yes

Abstract

A chemometrically optimized isolation procedure combined with gas chromatography-mass spectrometry detection technique has been proposed for quantitative determination of trace levels of nitrosamines in gastric juice samples of patients with the gastrointestinal tract problems. The extraction conditions of each nitrosamine were optimized using regression modelling based on central composite design. The extraction conditions for all nitrosamines were selected to be 10.7 min for extraction time, 4.2 for pH and 23 for 2-propanol percentage in extraction solution.