Calmodulin content and in vitro contractility of duodenum from streptozotocin-induced diabetic rats: Effects of insulin therapy and calmodulin antagonism

Ozturk Y., Aydin S., ÖZÇELİKAY A. T., Altan V., YildizogluAri N.

EUROPEAN JOURNAL OF PHARMACOLOGY, cilt.321, sa.1, ss.59-65, 1997 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 321 Sayı: 1
  • Basım Tarihi: 1997
  • Doi Numarası: 10.1016/s0014-2999(96)00928-4
  • Dergi Adı: EUROPEAN JOURNAL OF PHARMACOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.59-65
  • Anahtar Kelimeler: diabetic rat, streptozotocin, Ca2+, calmodulin, calmidazolium, insulin, intestinal reactivity, SMOOTH-MUSCLE CONTRACTION, BETA-ADRENERGIC RESPONSES, CALCIUM-BINDING PROTEINS, VASCULAR REACTIVITY, GASTROINTESTINAL-TRACT, LIGHT-CHAIN, MELLITUS, MANAGEMENT, CALPONIN, HYPERREACTIVITY
  • Ankara Üniversitesi Adresli: Evet

Özet

The effects of experimental diabetes and insulin treatment on the decreased reactivity of isolated rat duodenum to KCI and calmidazolium, a specific calmodulin antagonist, were examined. After 8 weeks of streptozotocin diabetes, the contractile effect of KCI and the non-competitive antagonistic effect of calmidazolium against KCI on isolated rat duodenum were decreased. Calmodulin levels, as measured by radioimmunoassay, were also found to be decreased in duodenum from streptozotocin-diabetic rats. Neither impaired reactivity to KCI nor decreased calmodulin levels in diabetic rat duodenum were corrected by treatment with insulin (10 IU/kg for 20 days). Following insulin treatment, there was only a partial correction in the antagonistic effect of calmidazolium as shown by the increase in non-competitive antagonist affinity constant.