Effect of the prostacyclin analogue, iloprost, on infarct size after permanent focal cerebral ischemia

Dogan A., Temiz C., Turker R., Egemen N., Baskaya M.

GENERAL PHARMACOLOGY-THE VASCULAR SYSTEM, vol.27, no.7, pp.1163-1166, 1996 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 27 Issue: 7
  • Publication Date: 1996
  • Doi Number: 10.1016/s0306-3623(96)00051-1
  • Journal Name: GENERAL PHARMACOLOGY-THE VASCULAR SYSTEM
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1163-1166
  • Keywords: focal cerebral ischemia, iloprost, prostacyclin, triphenyltetrazolium chloride, EARLY CONSEQUENCES, ARTERY OCCLUSION, NEURONAL INJURY, REPERFUSION, BRAIN, PROSTAGLANDINS, RAT, INHIBITORS, ZK-36374
  • Ankara University Affiliated: No

Abstract

This investigation was undertaken to study the effect of iloprost, a stable analogue of prostacyclin, on infarct size after permanent focal cerebral ischemia in the rabbit. Forty-two adult rabbits were subjected to left middle cerebral artery occlusion via the transorbital route. Fourteen rabbits received an intravenous (IV) infusion of 30 mu g/kg iloprost, 7 rabbits received an IV infusion of 10 mu g/kg, and 9 rabbits received an IV infusion of 20 mu g/kg iloprost. Twelve rabbits received an intravenous infusion of saline. Treatment with iloprost started immediately after middle cerebral artery occlusion and continued for 1 h. After killing the animals, brains were removed and five coronal slices were incubated in a 2,3,5-triphenyltetrazolium chloride solution to determine the infarct size. Treatment with 30 mu g/kg iloprost significantly reduced the infarct size compared with treatment with saline (3.49+/-2.79% vs. 9.03+/-4.26%, P<0.001), but the lower doses of iloprost did not have a beneficial effect on the size of the infarct. These results suggest that intravenous iloprost treatment after occlusion has a highly protective effect without any side effects such as hypotension. Copyright (C) 1996 Elsevier Science Inc.