STRAT-M® - TRANSDERMAL DIFFUSION TEST MEMBRANE: IN-VITRO PERMEATION STUDIES WITH DIFFERENT PENETRATION ENHANCERS

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Akhoroz B., Güler A., Öztürk Ö., İnal Ö., Badıllı F. U., Amasya Çelikten G.

14th International Symposium on Pharmaceutical Sciences (ISOPS), Ankara, Türkiye, 25 - 28 Haziran 2024

  • Yayın Türü: Bildiri / Yayınlanmadı
  • Basıldığı Şehir: Ankara
  • Basıldığı Ülke: Türkiye
  • Ankara Üniversitesi Adresli: Evet

Özet

STRAT-M® - TRANSDERMAL DIFFUSION TEST MEMBRANE: IN-VITRO PERMEATION STUDIES WITH DIFFERENT PENETRATION ENHANCERS

1Akhoroz B., 1,2Guler A., 1Ozturk O. 2Inal O., 2Badıllı U., 2Amasya G.

1 Ankara University, Graduate School of Health Sciences, Ankara, Turkey

2 Ankara University, Department of Pharmaceutical Technology, Ankara, Turkey, ayferguler@ankara.edu.tr

 

Introduction: Caffeine, an alkaloid compound, can promote hair growth, slow down the skin aging caused by UV radiation, and reduce cellulite by breaking down fat cells and improving blood circulation. This alkaloid is frequently used as hydrophilic model substance for human and animal skin permeation studies using Franz Diffusion cell with different synthetic membranes. This study aims to investigate the correlation between the in vitro permeation behavior of topical formulations through a transdermal diffusion test membrane (Strat-M® EMD Millipore, MA) and the classical regenerated cellulose membrane in the presence of Transcutol® and Tween 80® as penetration-enhancers. Strat-M® offers consistency, accessibility, and minimal variability for skin permeation studies in comparison with monolayer membranes (1).

 

Materials and Methods: 2% caffeine was used as a model active substance in 1% Carbopol 934® gels. Either Transcutol® or Tween 80® were added as enhancers in a ratio of 2%. Formulations were characterized in terms of viscosity, pH, mechanical properties and content uniformity. Then, in vitro permeation studies were carried out using Franz diffusion cells and caffeine amount were analyzed spectrophotometrically.

 

Results: The pH values of each formulation were compatible with the skin pH and their textural properties and viscosities were found suitable for topical application. Besides, content uniformity results show that caffeine was dispersed homogeneously into the gel formulations. 3.75% and 2.55% caffeine were found in receptor compartment when Strat-M® was used, while 45.30% and 46.9% caffeine release from regenerated cellulose membrane was observed for the gel formulations prepared using Tween 80 and Transcutol®, respectively.

 

Conclusions: As a result of this study, it was concluded that Strat-M® mimics the structure of skin better than classical synthetic membrane. There was no significant change in the pH and viscosity of the formulations as a result of two-month stability studies.

References:

1. Kaur, L., Singh, K., Paul, S., Singh, S., Singh, S., & Jain, S. K. (2018). AAPS PharmSciTech, 19(4), 1606-1624