Akademik Veri Yönetim Sistemi
South African Journal of Botany, cilt.186, ss.24-36, 2025 (SCI-Expanded)
Seaweeds are rich in macro and micro-nutrients, complex minerals, polysaccharides, vitamins and growth regulators such as gibberellins, auxins and cytokinins. Therefore, they have significant potential as an inexpensive natural bio-stimulant source in plant nutrition. In this study, the effect of seaweeds Jania rubens (L) Lamx and Cystoseira barbata (Stackhouse) C. Agardh extracts on micropropagation of important aquatic plants Anubias barteri nana (Engl.) Crusio and Alternanthera reineckii Brig. were investigated for the first time. Extracts of both seaweeds provided significant increases in micropropagation of both plant species without the use of plant growth regulators. Considering all tested MS (0, 1, 2 and 4 g/L) and seaweed extract (0, 2.5, 5, 10 and 20 %) concentrations, the highest rate of micropropagation in A. barteri and A. reineckii was achieved when 2.5 g/L J. rubens and C. barbata extracts were added to 4 g/L MS medium. In contrast, 20 % J. rubens and C. barbata extract added to 4 g/L MS medium significantly reduced the number of shoots per explant. This negative effect was less seen in nutrient media containing lower levels (1 and 2 g/L) of MS. In some media containing low MS, 20 % seaweed extract increased the number of shoots per explant compare to control MS medium. As in the number of shoots, the addition of J. rubens and C. barbata extracts to the nutrient media resulted in significant increases in root number, root length, diameter, fresh and dry weight of shoot clusters, chlorophyll a/b and total chlorophyll contents in both plant species. Well-rooted in vitro plantlets obtained by adding seaweed extract to the nutrient medium achieved 100% adaptation to aquariums.