Comparison between modified DNA extraction protocols and commercial isolation kits in grapevine (Vitis vinifera L.)

AKKURT M.

GENETICS AND MOLECULAR RESEARCH, vol.11, no.3, pp.2343-2351, 2012 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 11 Issue: 3
  • Publication Date: 2012
  • Doi Number: 10.4238/2012.august.13.8
  • Journal Name: GENETICS AND MOLECULAR RESEARCH
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.2343-2351
  • Keywords: Grapevine, DNA extraction, DNA isolation kits, NUCLEAR-DNA, PLANT
  • Ankara University Affiliated: No

Abstract

Various protocols have been developed and used for DNA extraction in grapevine. However, owing to the long duration of the isolation steps in previously developed protocols, researchers have preferred to use isolation kits for studies in recent years. In our study, the DNA yield and purity obtained using six methods - namely three DNA isolation protocols and three commercial DNA isolation kits - were compared. Modifications were made and the isolation steps were shortened in the previously developed DNA isolation protocols to achieve more rapid and practical protocols. The samples were taken from plants grown under vineyard and greenhouse conditions in two periods during spring and autumn. The best results among the six DNA isolation methods were discussed. The results were also supported with polymerase chain reaction analyses conducted with isolated DNAs.