In vitro effects of an azo compound on the haemolysis and unsaturated fatty acids of red blood cells

Pekiner B., Pennock J.

CLINICA CHIMICA ACTA, sa.2, ss.157-164, 1997 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Basım Tarihi: 1997
  • Doi Numarası: 10.1016/s0009-8981(97)00053-3
  • Dergi Adı: CLINICA CHIMICA ACTA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.157-164
  • Anahtar Kelimeler: haemolysis, red blood cell, 4,4'-azo-bis-(cyanovaleric acid), ALPHA-TOCOPHEROL LEVELS, LIPID-PEROXIDATION, HUMAN-ERYTHROCYTES, RADICAL INITIATOR, VITAMIN-E, HEMOLYSIS, MEMBRANE, HYDROPEROXIDES, INHIBITION, OXIDATION
  • Ankara Üniversitesi Adresli: Evet

Özet

Human red blood cells were treated with 4,4'-azo-bis-(4-cyanovaleric acid) (0-27 x 10(-3) M) in order to determine the effect of the compound on red brood cell haemolysis and unsaturated fatty acids. Maximum haemolysis amounting to approximately 100%, occurred after 60 min incubation with 15 x 10(-3) M azo compound and did not change to any significant extent by increasing incubation time to 4 h. The azo compound caused a decrease in unsaturated fatty acids unrelated to the number of double bonds. The percentage loss of unsaturated fatty acids was 60-100. Therefore the present study reveals that incubation of red blood cells with 15 x 10(-3) M 4,4'-azo-bis-(4-cyanovaleric acid) for 1 h causes maximum haemolysis. Also the damaging effect of the compound on red brood cell unsaturated fatty acids is parallel to haemolysis. These results shaw that this compound might have relevance for pathophysiology of red blood cells. (C) 1997 Elsevier Science B.V.