Molecular Methods for Detection of Invasive Fungal Infections and Mycobacteria and Their Clinical Significance in Hematopoietic Stem Cell Transplantation

Yurdakul P., Colakoglu S.

BONE MARROW AND STEM CELL TRANSPLANTATION, 2ND EDITION, cilt.1109, ss.239-270, 2014 (SCI-Expanded, Scopus) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 1109
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1007/978-1-4614-9437-9_13
  • Dergi Adı: BONE MARROW AND STEM CELL TRANSPLANTATION, 2ND EDITION
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.239-270
  • Anahtar Kelimeler: Hematopoietic stem cell transplantation, Aspergillosis, Candidiasis, Mycobacteria, Tuberculosis, Nucleic acid amplification tests, qPCR, IS6110, POLYMERASE-CHAIN-REACTION, NUCLEIC-ACID-AMPLIFICATION, REAL-TIME PCR, LINKED-IMMUNOSORBENT-ASSAY, MULTIDRUG-RESISTANT TUBERCULOSIS, BRONCHOALVEOLAR LAVAGE FLUID, BONE-MARROW-TRANSPLANTATION, CORD BLOOD TRANSPLANTATION, ASPERGILLUS-FUMIGATUS DNA, DIAGNOSTIC-ACCURACY
  • Ankara Üniversitesi Adresli: Evet

Özet

Infection remains an important source of morbidity and mortality in patients who undergo hematopoietic stem cell transplantation (HSCT). In the immune reconstitution period after transplantation, HSCT recipients are most likely to have bacterial or fungal infections. Invasive fungal infections (IFIs) and mycobacterial infections (MBIs) are among the complications of HSCT, with high morbidity and mortality rates. Early diagnosis of both is crucial in order to manipulate the disease and to avoid fulminant outcomes. This chapter reviews the current knowledge on the molecular diagnosis of IFIs and MBIs in HSCT recipients, describing two different polymerase chain reaction (PCR)-based methods, one commercial (qPCR, Roche) and one in-house IS6110-based protocol.