Analysis of cyst fluid antigens from different hosts for the serodiagnosis of hydatidosis

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Doganay A., GÖNENÇ B., SARIMEHMETOĞLU H. O., ÖGE H., ÖGE S., Adanir R., ...Daha Fazla

ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI, cilt.60, sa.1, ss.33-37, 2013 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 60 Sayı: 1
  • Basım Tarihi: 2013
  • Doi Numarası: 10.1501/vetfak_0000002550
  • Dergi Adı: ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.33-37
  • Anahtar Kelimeler: Cyst fluid antigen, diagnosis, donkey, mice, sheep, Western blotting, ECHINOCOCCUS-GRANULOSUS, ALVEOLAR ECHINOCOCCOSIS, ANTIBODIES, PROTEINS
  • Ankara Üniversitesi Adresli: Evet

Özet

The aims of this study were to determine the antigenic differences of hydatid cyst fluid from different animals and to investigate the effect of the origin of antigenic materials on the diagnosis of hydatidosis in humans and mice. For this purpose, the proteins of hydatid cyst fluid (HCF) isolated from the liver of sheep and donkeys and secondary hydatid cysts fluid (SHCF) from albino mice infected with protoscolices isolated from sheep and donkeys were separated by SDS-PAGE. This study was performed at the University of Ankara, Faculty of Veterinary Medicine, Department of Parasitology. For comparing antigenic profiles and detecting specific protein bands of those antigens, 10 infected and 10 non-infected human sera were tested for cystic echinococcosis (CE), and 16 positive and 9 negative mice sera were tested for secondary hydatidosis (SH) by using Western blotting technique. The specific protein band for mice sera was detected as 8 kDa (100%) for sheep's and donkey's hydatid cyst fluid. The specific proteins for human sera were detected at 24 lcDa (100%) and at 8 kDa (100%) for sheep's and donkey's hydatid cyst fluid respectively. No band was detected in negative mice and human sera. It was concluded that SHCF antigen from mice could be considered useful as being easy to provide and give good results for the diagnosis of CE.