The investigation of the presence of bovine herpesvirus type 4 (BoHV-4) in cows with metritis in a dairy herd

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BİLGE DAĞALP S., Gungor E., Demir A. B., Pinar-Muz D., YILMAZ V., OĞUZOĞLU T. Ç., ...Daha Fazla

ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI, cilt.57, sa.2, ss.87-91, 2010 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 57 Sayı: 2
  • Basım Tarihi: 2010
  • Doi Numarası: 10.1501/vetfak_0000002316
  • Dergi Adı: ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.87-91
  • Anahtar Kelimeler: Antibody, bovine herpesvirus type 4, metritis, polymerase chain reaction, sequence analysis, GLYCOPROTEIN-B, EXPERIMENTAL INOCULATION, VIRUS, VIRION
  • Ankara Üniversitesi Adresli: Evet

Özet

To investigate the occurrence of bovine herpesvirus type 4 (BoHV-4) infection in a dairy herd including cows with metritis, various methods were used for the diagnosis BoHV-4 in vaginal discharge samples. Blood serum samples were obtained from 368 cows for serodiagnosis of BoHV-4 infection. Of 368 serum samples, 256 (69.6%) were found positive for BoHV-4 specific antibodies. For the virological study, vaginal discharge samples of 55 cows with metritis were used and tested for BoHV-4 using polymerase chain reaction (PCR), virus isolation and immunofluorescence techniques. Twenty-nine percent (16/55) of the vaginal discharge samples obtained from cows with metritis were found positive for BoHV-4 DNA by PCR. In this study, the presence of BoHV-4 was determined in cows with metritis in post-partum period in a dairy herd.