The investigation of the presence of bovine herpesvirus type 4 (BoHV-4) in cows with metritis in a dairy herd

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BİLGE DAĞALP S., Gungor E., Demir A. B., Pinar-Muz D., YILMAZ V., OĞUZOĞLU T. Ç., ...More

ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI, vol.57, no.2, pp.87-91, 2010 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 57 Issue: 2
  • Publication Date: 2010
  • Doi Number: 10.1501/vetfak_0000002316
  • Journal Name: ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.87-91
  • Keywords: Antibody, bovine herpesvirus type 4, metritis, polymerase chain reaction, sequence analysis, GLYCOPROTEIN-B, EXPERIMENTAL INOCULATION, VIRUS, VIRION
  • Ankara University Affiliated: Yes

Abstract

To investigate the occurrence of bovine herpesvirus type 4 (BoHV-4) infection in a dairy herd including cows with metritis, various methods were used for the diagnosis BoHV-4 in vaginal discharge samples. Blood serum samples were obtained from 368 cows for serodiagnosis of BoHV-4 infection. Of 368 serum samples, 256 (69.6%) were found positive for BoHV-4 specific antibodies. For the virological study, vaginal discharge samples of 55 cows with metritis were used and tested for BoHV-4 using polymerase chain reaction (PCR), virus isolation and immunofluorescence techniques. Twenty-nine percent (16/55) of the vaginal discharge samples obtained from cows with metritis were found positive for BoHV-4 DNA by PCR. In this study, the presence of BoHV-4 was determined in cows with metritis in post-partum period in a dairy herd.