The Detection and Genetic Characterization Based on the S1 Gene Region of BCoVs from Respiratory and Enteric Infections in Turkey

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Alkan F., ÖZKUL A., Bilge-Dagalp S., Karaoglu T., OĞUZOĞLU T. Ç., Caliskan E., ...More

TRANSBOUNDARY AND EMERGING DISEASES, no.2, pp.179-185, 2011 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Publication Date: 2011
  • Doi Number: 10.1111/j.1865-1682.2010.01194.x
  • Journal Name: TRANSBOUNDARY AND EMERGING DISEASES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.179-185
  • Keywords: bovine, coronavirus, nested RT-PCR, S1 gene region, sequencing, AMINO-ACID-SEQUENCES, BOVINE CORONAVIRUS, SPIKE GLYCOPROTEIN, MOLECULAR ANALYSIS, WINTER DYSENTERY, CALF DIARRHEA, DAIRY-CATTLE, RT-PCR, TRACT, SUBUNIT
  • Ankara University Affiliated: Yes

Abstract

P>We investigated bovine coronavirus (BCoV) as an etiological agent in cattle with clinical respiratory and digestive signs using 147 feces and 199 nasal swab samples. A total of 18 test samples (16 feces and 2 nasal swap samples) were detected positive by ELISA and/or RT-PCR targeting the BCoV N gene. The partial S1 gene regions of BCoVs (An-4 and An-11) detected in feces samples from two herd-mate dairy calves were compared. Virological and serological results indicated that BCoVs are widespread in Turkey and are likely etiological agents in diarrhea cases in calves.