The power of High Impact Amplitude Manipulation (HIAM) technique for extracting the basic spectra of two Fixed-dose combinations (FDC) - Spectrophotometric purity analysis via spectral contrast angle

Saleh S. S. S., Lotfy H. M. M., TIRIS G., ERK N., El-Naem O. A. A.

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, cilt.273, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 273
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1016/j.saa.2022.121036
  • Dergi Adı: SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, Chemical Abstracts Core, Chimica, Compendex, EMBASE, INSPEC, MEDLINE, Veterinary Science Database
  • Anahtar Kelimeler: Ratio subtraction, Constant center, Constant extraction, Amlodipine, Atorvastatin, Candesartan, LIQUID-CHROMATOGRAPHY, BINARY-MIXTURE, CANDESARTAN CILEXETIL, AMLODIPINE BESYLATE, ATORVASTATIN
  • Ankara Üniversitesi Adresli: Evet

Özet

HIAM technique allows the extraction of the original constant signal of each single component out of interference signals of a mixture and further transformed into basic spectrum (D-0). It includes the methods: ratio subtraction coupled with unified constant subtraction (RS-UCS), constant center (CC) and constant extraction (CE). The technique was introduced for the analysis of two pharmaceutical formulations used to treat cardiovascular diseases. The formulations are binary combinations of Amlodipine (AML) with either Atorvastatin (ATR) or Candesartan (CND) which shows interefernce absorbance signals. The technique was valid over the linearity range of (5.0-35.0 mu g/ml) for AML, ATR and CND with recovery percentage 100.40 +/- 1.88 ,100.00 +/- 0.86 and 99.83 +/- 1.07, respectively . The extracted signals were tested for its purity by spectral contrast angle (cos theta) to illustrate the efficency of the HIAM technique where cos theta values ranges from (0.9902 to 0.9986). The presented technique was fully validated regarding ICH guidelines and were statistically compared using one-way ANOVA at 95% confidence. (C) 2022 Elsevier B.V. All rights reserved.