Adventitious shoot regeneration in sainfoin (Onobrychis viciifolia Scop.)

ÖZCAN S., YILDIZ M., SANCAK C., Özgen M.

Turkish Journal of Botany, cilt.20, sa.6, ss.497-501, 1996 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 20 Sayı: 6
  • Basım Tarihi: 1996
  • Dergi Adı: Turkish Journal of Botany
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.497-501
  • Anahtar Kelimeler: Adventitious shoot regeneration, Onobychis viciifolia, Organogenesis, Plant growth regulators, Sainfoin
  • Ankara Üniversitesi Adresli: Evet

Özet

Cotyledon and hypocotyl explants from in vitro grown seedlings of sainfoin were cultured on a range of media to develop an adventitious shoot regeneration system suitable for transformation. In general, callus formation was not influenced by the explants used, however, hypocotyls appeared to have a better regeneration capacity than cotyledons. The highest frequency of shoot formation was achieved from hypocotyl segments cultured onto the Murashige and Skoog (MS) medium containing 0.5 mg/l 6-benzylaminopurine (BAP) and 0.2 mg/l α-naphthaleneacetic acid (NAA). Regenerated shoots rooted in half strength MS supplemented with either 1 mg/l indole-butyric acid (IBA) or 1 mg/l NAA.