Adventitious shoot regeneration in sainfoin (Onobrychis viciifolia Scop.)
Turkish Journal of Botany, cilt.20, sa.6, ss.497-501, 1996 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 20 Sayı: 6
- Basım Tarihi: 1996
- Dergi Adı: Turkish Journal of Botany
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.497-501
- Anahtar Kelimeler: Adventitious shoot regeneration, Onobychis viciifolia, Organogenesis, Plant growth regulators, Sainfoin
- Ankara Üniversitesi Adresli: Evet
Özet
Cotyledon and hypocotyl explants from in vitro grown seedlings of sainfoin were cultured on a range of media to develop an adventitious shoot regeneration system suitable for transformation. In general, callus formation was not influenced by the explants used, however, hypocotyls appeared to have a better regeneration capacity than cotyledons. The highest frequency of shoot formation was achieved from hypocotyl segments cultured onto the Murashige and Skoog (MS) medium containing 0.5 mg/l 6-benzylaminopurine (BAP) and 0.2 mg/l α-naphthaleneacetic acid (NAA). Regenerated shoots rooted in half strength MS supplemented with either 1 mg/l indole-butyric acid (IBA) or 1 mg/l NAA.