Electromembrane extraction of tramadol from exhaled breath condensate and its liquid chromatographic analysis

Hoseininezhad-Namin M. S., Seyfinejad B., Ozkan S. A., Soleymani J., Khoubnasabjafari M., Jouyban-Gharamaleki V., ...Daha Fazla

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, cilt.219, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 219
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1016/j.jpba.2022.114959
  • Dergi Adı: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Analytical Abstracts, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Chimica, EMBASE, International Pharmaceutical Abstracts, MEDLINE, Veterinary Science Database
  • Anahtar Kelimeler: HPLC-UV, Electromembrane extraction, Tramadol, Exhaled breath condensate, SOLID-PHASE MICROEXTRACTION, HUMAN PLASMA, MASS-SPECTROMETRY, SPECTROPHOTOMETRIC DETERMINATION, O-DESMETHYLTRAMADOL, BIOLOGICAL-FLUIDS, WATER SAMPLES, ORAL FLUID, URINE, DRUGS
  • Ankara Üniversitesi Adresli: Evet

Özet

Tramadol has extracted from the exhaled breath condensate (EBC) samples through the supported liquid membrane consisting of 2-nitrophenyl octyl ether impregnated in the hollow fiber wall, and the lumen of the hollow fiber was filled with 20 mu L of an acceptor phase. Under the optimum conditions of the electromembrane extraction, i.e. the stirring speed of 750 rpm, extraction time of 20 min, acceptor pH at 1.0, donor phase pH at 6.0, and an applied voltage of 170 V across the supported liquid membrane, a preconcentration factor of 128-fold with a extraction recovery of 64% was achieved. Acceptable linearity was obtained in the tramadol concentration range of 5-1000 ng mL(-1) (R-2 = 0.9999) with a limit of detection of 1.5 ng mL(-1) and a limit of quantitation of 5 ng mL(-1). The relative standard deviations for the intra-day and inter-day replications were obtained between 0.4% and 2.5%. The validated technique was successfully used to determine tramadol in real EBC samples.