Akademik Veri Yönetim Sistemi
Journal of B.U.ON., cilt.14, sa.4, ss.647-652, 2009 (SCI-Expanded)
Purpose: To investigate the effects of vitamin C and N-acetylcysteine (NAC) against cyclophosphamide (CP) -inducedgeno toxic damage in exfoliated bladder cells of mice by micronucleus (MN) assay. Methods: For each experimental step, 6-8 Swiss albino balb/c male mice were used. CP was used as positive control. Vitamin C (10, 30 and 60 mg/kg) and CP (51.6 mg/kg) were administered intraperitoneally to the experimental animals. Vitamin C was administered twice, one dose 24 h prior to the CP administration and the second dose simultaneously with the CP. NAC (200, 400 and 800 mg/kg) was administered by gavage for 7 consecutive days before the injection of CP. Distilled water and normal saline as negative controls I and II were used, respectively. Ten days after CP treatment, the mice were sacrificed and bladders were isolated and cut, and exfoliated cells were scraped from the bladder walls. Air-dried smears were stained by Feulgen reaction. MN frequencies were scored in 1000 epithelial cells per animal and defined as MN per thousand (%o). Results: Three doses of vitamin C (10, 30 and 60 mg/kg) showed a significant inhibitory effect on MN frequencies in mouse bladder cells when compared with those of positive control group (p < 0.05). Dose-dependent inhibitory effect of vitamin C was observed only between the doses of 10 and 60 mg/kg (p < 0.05). Histopathological changes that depended on CP- induced inflammatory infiltration and haemorrhage in mucosa propria were not observed in all 3 vitamin C doses. Three doses of NAC (200, 400 and 800 mg/kg) inhibited the CP-inducedgenotoxicity(p < 0.05), however, the antigenotoxic effect of NAC was not dose-dependent. Histopathological changes that depended on CP-induced inflammatory infiltration and haemorrhage in mucosa propria were not observed in 200 and 400 mg/kg NAC dosage. The extent of desquamation in bladder was similar in all 3 doses of NAC when compared with the positive control group. Conclusion: Our study indicated that vitamin C and NAC reduced the CP-induced MN frequencies in target (bladder) cells of mice by 41-71% in all cases. The modifying effects of vitamin C and NAC against CP-inducedgeno toxic damage may be due to their antioxidant, nucleophilic properties and to the ability to act as precursors of glutathione. © 2009 Zerbinis Medical Publications.