The stability studies on ALT, LDH and GGT enzymes in control sera used at clinical biochemistry laboratories Klinik biyokimya laboratuvarlarında kullanılan kontrol serumlarında, ALT, LDH ve GGT enzimlerine i̇lişkin stabilite çalışmaları


Hişmioǧullari A. A., SEL T.

Kafkas Universitesi Veteriner Fakultesi Dergisi, cilt.17, sa.3, ss.451-456, 2011 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 17 Sayı: 3
  • Basım Tarihi: 2011
  • Dergi Adı: Kafkas Universitesi Veteriner Fakultesi Dergisi
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.451-456
  • Anahtar Kelimeler: Enzyme, Liver function test, Quality control, Stability
  • Ankara Üniversitesi Adresli: Evet

Özet

Summary The purpose of the current study is to make a stability work about some liver's function parameters such as alanine transaminase (ALT; EC:2.6.1.2), L-lactate dehydrogenase (LDH; EC:1.1.1.27) and γ-glutamyltransferase (GGT; EC:2.3.2.2.) enzymes that are present in five control sera belonging to diff erent brands and suppliers. The stability of enzymes stored for a specified period at 4°C, 25°C and -30°C storage conditions, their activities were measured in autoanalyser accordance with the manufacturer's instructions for use in the commercial kit and periodic intervals. The control sera stored at 4°C, belonging to one brand, the GGT activity decreased at the rate of 21.78% in 72 h. Similarly, the control serum stored at 25°C, belonging to one brand, concerning the same parameters, the activity decreased at the rate of 19.49% as well. The control sera stored at 4°C, belonging to two brands, the LDH activities, decreased at the rate of 28.04% and 42.17% in 72 h. Similarly, the control sera stored at 25°C, belonging to three brands, concerning the same parameters, the activities decreased at the rate of 40%, 25.36% and 40.56% in 72 h. The stabilities of liver's parameters in control sera, stored at -30°C for 96 hour and at 4°C for 72 hour were satisfactory, generally. On the other hand, the stabilities of liver's parameters in control sera, stored at 25°C for 72 h were unsatisfactory in large numbers.