Hinf-I digestion of cytochrome oxidase I region is not a diagnostic test for A-m. lamarckii

Kandemir I., Pinto M. A., Meixner M. D., Sheppard W. S.

GENETICS AND MOLECULAR BIOLOGY, vol.29, no.4, pp.747-749, 2006 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 29 Issue: 4
  • Publication Date: 2006
  • Doi Number: 10.1590/s1415-47572006000400027
  • Journal Name: GENETICS AND MOLECULAR BIOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.747-749
  • Keywords: mtDNA, cytochrome oxidase I, Apis mellifera lamarckii, O lineage, HONEY-BEE HYMENOPTERA, MITOCHONDRIAL-DNA, 4TH LINEAGE, IDENTIFICATION, POPULATIONS
  • Ankara University Affiliated: No

Abstract

Restriction fragment length polymorphism of whole mitochondrial DNA or PCR amplified mtDNA regions are known to be useful in discriminating among honey bee lineages and also some individual subspecies. In this study, PCR-amplified fragments of cytochrome oxidase I(CO-I) and cytochrome B (Cyt B) of honey bees sampled from different countries (Cyprus, Turkey, Ethiopia, Syria and Egypt) were digested with Hinf I and Bgl II restriction enzymes, respectively. Eastern Europe and Mediterranean honey bee subspecies were separated by the Cyt B/Bgl II analysis, although Hinf I digestion of the CO-I region yielded much finer resolution within different honey bee lineages. Here we report that CO-I/Hinf-I is a discriminative test for the mitochondrial "O" lineage, rather than a diagnostic site for A. m. lamarckii.