Hindy O. A., Pınarbaşı B., Bakıcı M., Demirtaş O. B., Büyüksungur A., Orhan K., ...Daha Fazla
TERMIS EU, Freiburg, Almanya, 20 - 23 Mayıs 2025, cilt.31, sa.11, ss.489-588, (Özet Bildiri)
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Yayın Türü:
Bildiri / Özet Bildiri
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Cilt numarası:
31
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Doi Numarası:
10.1089/ten.tea.2025.90912.abstracts.parta
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Basıldığı Şehir:
Freiburg
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Basıldığı Ülke:
Almanya
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Sayfa Sayıları:
ss.489-588
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Ankara Üniversitesi Adresli:
Evet
Özet
Introduction/Objectives
In situ bioprinting of bone represents a transformative approach in the field of regenerative medicine to directly repair and regenerate bone tissues at the injury site. Unlike traditional bone grafting methods, in situ bioprinting offers the advantage of real-time customization, enabling tailored scaffolds that conform to the unique geometry of each defect and integrate seamlessly with surrounding native tissues.
Recent advancements in handheld bioprinting devices have showcased an innovative approach for in situ applications where precision is less critical than adaptability. While handheld bioprinters can accommodate irregular defect shapes, their output may vary due to manual control, making them less suitable for situations where exact spatial arrangement and complex structures are essential.
The use of in situ bioprinting with a traditional 3D Bioprinter device is particularly advantageous in terms of accuracy and practicality, in complex or irregularly shaped bone defects- which are difficult to treat using conventional methods. Furthermore, in situ bioprinting minimizes the risk of contamination and reduces surgical time, making it a promising approach for point-of-care applications in orthopedic surgery.
Methods
In this study, critical-sized bone defects were created on the parietal bones of rabbits. An autologous adipose-derived stem cell-laden alginate/hydroxyapatite bioink was applied using a 3D bioprinter (Envisiontech Bioplotter) to the defects in the animals under anesthesia. Control groups were applied as non-cell bioink and sham. Post-operative evaluations included micro-CT scanning and histopathological analysis to assess bone healing and bone-material integration.
Results
The results demonstrated successful bone regeneration with the in situ bioprinting approach, as compared to the sham group and the group using bioink-only. Quantitative analyses in micro CT revealed that the cellular group had the highest bone volume and percent bone volume, followed by the acellular group, and the sham. The bone surface/volume ratio and bone surface density were higher in both the cellular and acellular groups compared to the sham, indicating better bone formation and coverage.
In defects filled with acellular material, a thin capsule structure was observed around the material, primarily consisting of fibrocytes. Additionally, periosteal proliferations were seen extending into and around the material, indicating a response to the implanted scaffold. In contrast, defects filled with cellular material exhibited a thicker capsule structure, primarily composed of fibroblasts and collagen fibers. This group showed numerous segmented and/or pyknotic neutrophils on the capsule and severe inflammatory cell infiltration immediately outside the capsule, consisting of lymphocytes, plasma cells, and a few macrophages. In the sham, primarily fibrocytes and collagen fibers were observed, suggesting an attempt at repair rather than proper bone regeneration.
Conclusions
The novelty of this research lied in the direct application of bioprinting onto live animals under anesthesia, which enhances the clinical relevance of the findings and paves the way for future clinical applications.