Bacterial membrane vesicle functions, laboratory methods, and applications

AYTAR ÇELİK P., DERKUŞ B., Erdogan K., Barut D., Manga E. B., Yildirim Y., ...Daha Fazla

BIOTECHNOLOGY ADVANCES, cilt.54, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Derleme
  • Cilt numarası: 54
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1016/j.biotechadv.2021.107869
  • Dergi Adı: BIOTECHNOLOGY ADVANCES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Aerospace Database, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Communication Abstracts, Compendex, EMBASE, Environment Index, Geobase, INSPEC, MEDLINE, Metadex, Pollution Abstracts, Veterinary Science Database, Civil Engineering Abstracts
  • Anahtar Kelimeler: Bacterial extracellular vesicle, Downstream technology, Cancer therapy, Vaccine, Delivery system, Bacterial outer membrane vesicle, HORIZONTAL GENE-TRANSFER, OUTER-MEMBRANE, EXTRACELLULAR VESICLES, HYPERTHERMOPHILIC ARCHAEA, PROTEIN EXPRESSION, CARGO PROTEINS, WESTERN-BLOT, CELL, DNA, BIOGENESIS
  • Ankara Üniversitesi Adresli: Evet

Özet

Bacterial membrane vesicles (BMVs) are cupped-shaped structures formed by bacteria in response to environmental stress, genetic alteration, antibiotic exposure, and others. Due to the structural similarities shared with the producer organism, they can retain certain characteristics like stimulating immune responses. They are also able to carry molecules for long distances, without changes in the concentration and integrity of the molecule. Bacteria originally secrete membrane vesicles for gene transfer, excretion, cell to cell interaction, pathogenesis, and protection against phages. These functions are unique and have several innovative applications in the pharmaceutical industry that have attracted both scientific and commercial interest.This led to the development of efficient methods to artificially stimulate vesicle production, purification, and manipulation in the lab at nanoscales. Also, for specific applications, engineering methods to impart pathogen antigens against specific diseases or customization as cargo vehicles to deliver payloads to specific cells have been reported. Many applications of BMVs are in cancer drugs, vaccines, and adjuvant development with several candidates in clinical trials showing promising results. Despite this, applications in therapy and commercialization stay timid probably due to some challenges one of which is the poor understanding of biogenesis mechanisms. Nevertheless, so far, BMVs seem to be a reliable and cost-efficient technology with several therapeutic applications. Research toward characterizing more membrane vesicles, genetic engineering, and nanotechnology will enable the scope of applications to widen. This might include solutions to other currently faced medical and healthcare-related challenges.