Cryogenic X-ray crystallographic studies of biomacromolecules at Turkish Light Source “Turkish DeLight”
Turkish Journal of Biology, vol.47, no.1, 2023 (SCI-Expanded, Scopus, TRDizin)
- Publication Type: Article / Article
- Volume: 47 Issue: 1
- Publication Date: 2023
- Doi Number: 10.55730/1300-0152.2637
- Journal Name: Turkish Journal of Biology
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, Veterinary Science Database, TR DİZİN (ULAKBİM)
- Keywords: atomic resolution, drug development, drug repurposing, light source, structural biology, structural dynamics, X-ray crystallography
- Ankara University Affiliated: Yes
Abstract
X-ray crystallography is a robust and powerful structural biology technique that provides high-resolution atomic structures of biomacromolecules. Scientists use this technique to unravel mechanistic and structural details of biological macromolecules (e.g., proteins, nucleic acids, protein complexes, protein-nucleic acid complexes, or large biological compartments). Since its inception, single-crystal cryocrystallography has never been performed in Türkiye due to the lack of a single-crystal X-ray diffractometer. The X-ray diffraction facility recently established at the University of Health Sciences, İstanbul, Türkiye will enable Turkish and international researchers to easily perform high-resolution structural analysis of biomacromolecules from single crystals. Here, we describe the technical and practical outlook of a state-of-the-art home-source X-ray, using lysozyme as a model protein. The methods and practice described in this article can be applied to any biological sample for structural studies. Therefore, this article will be a valuable practical guide from sample preparation to data analysis.