Akademik Veri Yönetim Sistemi
Journal de Pharmacie de Belgique, cilt.53, sa.3, ss.180, 1998 (SCI-Expanded)
Two new spectrophotometric methods,ratio spectra derivative spectrophotometry and Vierordt's method for the determination of triamterene and hydrochlorothiazide in a mixture were described. These procedures do not require any separation step. In the first method ,derivative amplitudes were measured in their first derivative of the ratio spectra of their mixture solution in methanol:0.1 N HCl (1:1) at 284.4 nm for triamterene and at 278.6 nm for hydrochlorothiazide. Calibration graphs were linear in the range 5.0-50.0 μg.ml-1 for triamterene and 2.5-25.0 μg.ml-1 for hydrochlorothiazide. In the second method, the relative standard deviations were found as 1.51 % and 0.82 % for triamterene and hydrochlorothiazide , respectively. In the Vierordt's method, A11 (1 % , 1 cm) values for triamterene and hydrochlorothiazide were determined at 380.0 nm and 319.4 nm in their zero-order spectra in methanol : 0.1 N HCl (1:1). The amounts of both compounds were calculated by means of equations with two unknowns using the values. The relative standard deviations in Vierordt's method for triamterene and hydrochlorothiazide were found to be 0.50 % and 0.98 %, respectively. The methods were successfully applied to a pharmaceutical formulation for the determination of these compounds.