Akademik Veri Yönetim Sistemi
Microchemical Journal, cilt.218, 2025 (SCI-Expanded)
In this study, we report the fabrication of the first electrochemical sensor for the tyrosine kinase inhibitor anticancerous drug, Nintedanib. The integration of the molecular imprinting approach with suitable electrode modifications enabled the selective and sensitive determination of the drug. Upon a glassy carbon electrode modified with a ruthenium complex and multi-walled carbon nanotube, a molecularly imprinted polymer layer was electropolymerized from the monomer o-phenylenediamine and Nintedanib solution. The experimental parameters during the extraction and rebinding of the template were monitored utilizing a redox marker, [Fe(CN)6]3−/4– through cyclic voltammetric and differential pulse voltammetric techniques. The characterization of the synthesized molecularly imprinted polymer was done by methods such as Fourier transform infrared spectroscopy and scanning electron microscopy. The images revealed a distinct morphology for the molecularly imprinted polymer compared to the non-imprinted polymer and the polymer containing the ruthenium pincer complex and multi-walled carbon nanotube. From the calibration curve, analytical parameters, including the linear range, limit of detection, and limit of quantification, were found to be 100 pM to 5 nM, 18 pM, and 56 pM, respectively. Moreover, the sensor was successfully applied for the detection of the drug in a pharmaceutical dosage form as well as in a synthetic serum sample. The capability of the sensor to differentiate the target from structurally similar classes of anticancer drugs was tested. Moreover, the greenness of the method was assessed using the Analytical GREEnness Metric and the AGREEMIP tool, which evaluates sample preparation with molecularly imprinted polymers.