Preparation of methacrylamide grafted and dye-ligand immobilized PET fibers: Studies of adsorption and purification of lysozyme

KARAKIŞLA M., Bayramoglu G., Arica M. Y.

JOURNAL OF APPLIED POLYMER SCIENCE, cilt.108, sa.5, ss.3313-3323, 2008 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 108 Sayı: 5
  • Basım Tarihi: 2008
  • Doi Numarası: 10.1002/app.27698
  • Dergi Adı: JOURNAL OF APPLIED POLYMER SCIENCE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.3313-3323
  • Anahtar Kelimeler: PET fibers, methacrylamide, graft copolymerization, dye ligand, adsorption, lysozyme, isotherm model, kinetics, METAL AFFINITY-CHROMATOGRAPHY, POLY(ETHYLENE-TEREPHTHALATE) FIBERS, PROTEIN ADSORPTION, BENZOYL-PEROXIDE, STATIONARY PHASES, COLLAGEN-FIBER, EGG-WHITE, MEMBRANES, SEPARATION, BEADS
  • Ankara Üniversitesi Adresli: Evet

Özet

In this study, novel affinity chromatographic fibers was prepared from methacrylamide grafted poly(ethylene terephthalate), PET-g-pMAA, using benzoyl-peroxide as an initiator. A dye ligand (i.e., Procion Brown) as a ligand was then covalently immobilized on the different amount of pMAAm grafted PET fibers, (PET-g-pMAAm-PB). The fibers were characterized by surface area measurement, infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), and scanning electron microscopy (SEM). Adsorptive properties of the composite fibers were tested using a model protein (i.e., lysozyme). To achieve these purposes, the influence of pH, ionic strength, initial lysozyme concentration, and temperature on adsorption system has been investigated and evaluated. A maximum lysozyme adsorption PET-g-pMAAm-PB fiber was obtained as 43.9 mg g(-1) at pH 7.5. The experimental equilibrium data obtained for lysozyme adsorption onto PET-g-pMAAm-PB fibers fitted well to the Langmuir isotherm model. The result of kinetic analyzed for lysozyme adsorption onto affinity fibers showed that the second-order rate equation was favorable. The purity of the eluted lysozyme, as determined by HPLC, was 84% with recovery 73% for PET-g-pMAAm-PB fiber. Experiments on regeneration and dynamic adsorption were also performed. It appears that PET-g-pMAAm-PB fibers can be applied for lysozyme separation without causing any denaturation. (C) 2008 Wiley Periodicals, Inc.