Akademik Veri Yönetim Sistemi
7th International Conference on Advances in Natural and Applied Sciences (ICANAS 2024) , Antalya, Türkiye, 17 - 20 Nisan 2024, ss.147-148, (Özet Bildiri)
Wound healing is a type of regeneration involving 4 consecutive stages: haemostasis,
inflammation, proliferation and remodeling. Occuring mainly in proliferative phase
vasculature formation is a vital step for wound healing. Induced pluripotent stem cells
(iPSCs) have invaluable capacity for tissue regeneration and wound healing because of
their ability to differentiate into multiple cell lineages, lack of immune rejection and
ethical concerns.
Studies in the literature could be divided to 4 groups: cellular differentiation based,
exosome based, scaffold-biomaterials based and gene based studies. First group of
studies involve differentiation of iPSCs to smooth muscle cells (SMCs), epithelial cells
(ECs), keratinocytes. Mainly these iPSCs derived SMCs enhanced wound healing via
angiogenesis by increasing growth factors and cytokines such as bFGF, VEGF (1, 2),
cellular proliferation, survival of hiPSCs and macrophage polarization (2), collagen
deposition, macrophage infiltration (3), increased blood vessel density and growth
factors (4).
iPSCs derived exosomes-microvesicles improved wound healing by improved collagen I
and III levels, reduced inflammation (5), thickening epidermis and increased collagen
deposition and α-smooth muscle actin (α-SMA) together with CD31 (6), increased
number of nerve fibers and α-SMA and CD31 (7). In another study they increased wound
healing by improved collagen-elastin deposition and vessel density (8).
Collagen scaffolds with hIPSCs derived SMCs healed wound by epidermal and dermal
thickening, enhanced cytokines and growth factors (9). Human skin substitute with
fibroblasts and keratinocytes improved wound healing by neovascularization, and blood
vessel invasion from wound bed (10).
When integrin β1 was knocked out in iPSCs, it resulted with improved iPSCs migration
and survival, angiogenesis, blood perfusion and consequently wound healing (11).