Regulation of the ribonucleotide reductases in Lactococcus lactis subsp cremoris

Akyol İ.

ACTA BIOLOGICA HUNGARICA, cilt.58, sa.1, ss.105-114, 2007 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 58 Sayı: 1
  • Basım Tarihi: 2007
  • Doi Numarası: 10.1556/abiol.58.2007.1.10
  • Dergi Adı: ACTA BIOLOGICA HUNGARICA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.105-114
  • Anahtar Kelimeler: FlpA, FlpB, glutaredoxin, Lactococcus lactis, NrdH, proteomics, GRAM-POSITIVE BACTERIA, FNR-LIKE PROTEINS, ESCHERICHIA-COLI, SYSTEM, GENE, GLUTAREDOXIN, THIOREDOXIN, PLASMID, ENZYME, FUSION
  • Ankara Üniversitesi Adresli: Hayır

Özet

Lactococcus lactis has two essential ribonucleotide reductases for DNA biosynthesis and repair which are affected in the presence or absence of oxygen. Expression of glutaredoxin like protein (NrdH), the hydrogen donor for ribonucleotide reductase, was found to be regulated by the FNR like proteins (FIpA and FlpB). Proteomics Study demonstrated that expression level of NrdH significantly decreased in the flpA and flpAB deletion mutants. The nrdH gene is located in an nrdHIEF operon and encoding the NrdEF ribonucleotide reductase, which is active under aerobic and anaerobic conditions. Regulation of expression of the nrdHIEF operons was investigated using beta-galactosidase as a reporter gene. The 588 bp fragment containing the nrdH promoter and gene cloned into the pORI vector immediately upstream of a promoterless lacZ gene. Constructed plasmid was transferred into wild type (MG1363), single mutant (flpA or flpB) and double mutant (flpAB). Aerobically, nrdH promoter activity is 15-fold higher than anaerobic expression.