Akademik Veri Yönetim Sistemi
VI. International Agricultural, Biological & Life Science Conference, Antalya, Türkiye, 30 Ekim - 01 Kasım 2024, cilt.1, sa.1, ss.125
Plant AT-rich sequence and zinc-binding (PLATZ) proteins belong to a novel class of plantspecific zinc-finger-dependent DNA-binding proteins that play essential roles in plant growth and development and abiotic stress responses. While the PLATZ (PvPLATZ) gene family has been identified in various species, a comprehensive identification and characterization of this gene family in Phaseolus vulgaris L., a nutritionally significant edible crop, has not yet been performed. In this study, using a range of bioinformatics and in-silico analyses, 15 PvPLATZ genes were identified in the P. vulgaris genome. In silico analyses of gene structures, protein properties, phylogenetic relationships, and various other characteristics of PvPLATZ members were conducted. The molecular weights of putative PvPLATZ proteins ranged from 21.99 to 28.46 kDa and the intron numbers of PvPLATZ genes ranged from 2 to 3. The physical locations of PvPLATZ genes were further examined to reveal their distribution across different chromosomes. In addition to structural characterization, we conducted promoter region analysis to predict potential cis-regulatory elements involved in stress response and gene expression regulation. Phylogenetic analysis revealed the evolutionary relationships between P. vulgaris PvPLATZ genes and those in other plant species, highlighting conserved and unique features. Furthermore, miRNA target analysis identified potential miRNAs that may regulate PvPLATZ gene expression, suggesting a post-transcriptional regulatory layer. Gene ontology (GO) analysis provided a comprehensive overview of the predicted biological processes, molecular functions, and cellular components associated with the identified PvPLATZ genes. In addition, RNA-seq data was utilized to carry out a comparative expression analysis of PvPLATZ genes. According to RNA-seq analysis, all genes were up- or down-regulated except for PvPLATZ-8 and PvPLATZ-13 after salt and drought stress treatment of P. vulgaris. Overall, the results indicated that certain PvPLATZ genes may play significant roles in abiotic stress responses in P. vulgaris. This study lays the foundation for future research aimed at elucidating the molecular mechanisms underlying PvPLATZ-mediated stress responses and developing stressresilient crop varieties.