FAST DETERMINATION OF DIPHENHYDRAMINE HYDROCHLORIDE IN RECONSTITUTABLE SYRUPS BY CWT, PLS AND PCR METHODS

DEVRİM GÖKBERK B., DİNÇ E., BOZKIR A.

ACTA POLONIAE PHARMACEUTICA, cilt.71, sa.5, ss.721-729, 2014 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 71 Sayı: 5
  • Basım Tarihi: 2014
  • Dergi Adı: ACTA POLONIAE PHARMACEUTICA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.721-729
  • Anahtar Kelimeler: diphenhydramine hydrochloride, reconstitutable syrup formulation, continuous wavelet transform, quantitative analysis, CONTINUOUS WAVELET TRANSFORMS, NONAQUEOUS CAPILLARY-ELECTROPHORESIS, LIQUID-CHROMATOGRAPHIC METHOD, PHARMACEUTICAL-PREPARATIONS, TERNARY MIXTURE, BINARY-MIXTURES, TABLETS, ELECTROCHEMILUMINESCENCE, SPECTROPHOTOMETRY, PSEUDOEPHEDRINE
  • Ankara Üniversitesi Adresli: Evet

Özet

Diphenhydramine hydrochloride (DPH), a histamine H1-receptor antagonist, is widely used as antiallergic, antiemetic and antitussive drug found in many pharmaceutical preparations. In this study, a new reconstitutable syrup formulation of DPH was prepared because it is more stable in solid form than that in liquid form. The quantitative estimation of the DPH content of a reconstitutable syrup formulation in the presence of pharmaceutical excipients, D-sorbitol, sodium citrate, sodium benzoate and sodium EDTA is not possible by the direct absorbance measurement. Therefore, a signal processing approach based on continuous wavelet transform was used to determine the DPH in the reconstitutable syrup formulations and to eliminate the effect of excipients on the analysis. The absorption spectra of DPH in the range of 5.0-40.0 mu g/mL were recorded between 200-300 nm. Various wavelet families were tested and Biorthogonal1.1 continuous wavelet transform (BIOR1.1-CWT) was found to be optimal signal processing family to get fast and desirable determination results and to overcome excipient interference effects. For a comparison of the experimental results obtained by partial least squares (PLS) and principal component regression (PCR) methods were applied to the quantitative prediction of DPH in the mentioned samples. The validity of the proposed BIOR1.1-CWT, PLS and PCR methods were achieved analyzing the prepared samples containing the mentioned excipients and using standard addition technique. It was observed that the proposed graphical and numerical approaches are suitable for the quantitative analysis of DPH in samples including excipients.