HPLC method for the analysis of paracetamol, caffeine and dipyrone

ALTUN M. L.

TURKISH JOURNAL OF CHEMISTRY, vol.26, no.4, pp.521-528, 2002 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 26 Issue: 4
  • Publication Date: 2002
  • Journal Name: TURKISH JOURNAL OF CHEMISTRY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.521-528
  • Keywords: paracetamol, acetaminophen, caffeine, dipyrone, high performance liquid chromatography, validation, ASCORBIC-ACID, PHARMACEUTICAL PREPARATIONS, ACETAMINOPHEN, BIOAVAILABILITY, SODIUM, TABLET
  • Ankara University Affiliated: Yes

Abstract

An accurate. simple, reproducible and sensitive method for the determination of paracetamol. caffeine and dipyrone was developed and validated. Paracetamol, caffeine and dipyrone were separated using a mu-Bondapack C-8 column by isocratic elution with a flow rate of 1.0 ml/min. The mobile phase composition was 0.01 M KH2 PO4-methanol-acetonitrile-isopropyl alcohol (420: 20 30: 30) and spectrophotometric detection was carried out at 215 nm. The linear range of determination for paracetamol, caffeine and dipyrone were 0.409-400 mug/ml, 0.151-200 mug/ml and 0.233-600 mug/ml. respectively. The method was shown to be linear, reproducible, specific, sensitive and rugged.