Determination of alpha naphthyl acetate esterase activity in the peripheral blood leukocytes of ostrich (Struthio camelus masaicus)

Ergun E., Ergun L., Ozen A., Asti R.

Revue de Medecine Veterinaire, cilt.155, sa.3, ss.147-150, 2004 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 155 Sayı: 3
  • Basım Tarihi: 2004
  • Dergi Adı: Revue de Medecine Veterinaire
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.147-150
  • Anahtar Kelimeler: leukocyte, T lymphocyte, ANAE activity, ostrich, T-LYMPHOCYTES, ACID ESTERASE, IDENTIFICATION, MARKER, TISSUES, CHICKEN, LIGHT
  • Ankara Üniversitesi Adresli: Evet

Özet

The aims of this study were to determine the alpha naphthyl acetate esterase (ANAE) activity in peripheral blood leukocytes and the ratio of T lymphocytes in healthy adult Ostriches. Blood smears from 20 adult and healthy Ostriches were tested for detecting ANAE activity by a specific staining procedure. Thrombocytes, heterophils and eosinophile granulocytes gave negative reaction, whereas monocytes showed a diffuse positivity. On the contrary, the pattern of ANAE in T lymphocytes was granular, with 1 or 2, sometimes more, red granules. In blood, the percent of ANAE positive lymphocytes was 59.3 ± 0.40 %. In conclusion, ANAE activity showed a specific granular expression in T lymphocytes in Ostriches and allowed to determine directly and quickly the ratio of circulationg T lymphocytes.