Determination of alpha naphthyl acetate esterase activity in the peripheral blood leukocytes of ostrich (Struthio camelus masaicus)
Revue de Medecine Veterinaire, cilt.155, sa.3, ss.147-150, 2004 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 155 Sayı: 3
- Basım Tarihi: 2004
- Dergi Adı: Revue de Medecine Veterinaire
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.147-150
- Anahtar Kelimeler: leukocyte, T lymphocyte, ANAE activity, ostrich, T-LYMPHOCYTES, ACID ESTERASE, IDENTIFICATION, MARKER, TISSUES, CHICKEN, LIGHT
- Ankara Üniversitesi Adresli: Evet
Özet
The aims of this study were to determine the alpha naphthyl acetate esterase (ANAE) activity in peripheral blood leukocytes and the ratio of T lymphocytes in healthy adult Ostriches. Blood smears from 20 adult and healthy Ostriches were tested for detecting ANAE activity by a specific staining procedure. Thrombocytes, heterophils and eosinophile granulocytes gave negative reaction, whereas monocytes showed a diffuse positivity. On the contrary, the pattern of ANAE in T lymphocytes was granular, with 1 or 2, sometimes more, red granules. In blood, the percent of ANAE positive lymphocytes was 59.3 ± 0.40 %. In conclusion, ANAE activity showed a specific granular expression in T lymphocytes in Ostriches and allowed to determine directly and quickly the ratio of circulationg T lymphocytes.