Functional characteristics of bovine adrenal chromaffin cells on 3D- Plga scaffolds: Implications for tissue engineering

Elçin Y. M., Elçin A. E., Pappas G. D.

Second Smith and Nephew International Symposium - Tissue Engineering 2000: Advances in Tissue Engineering, Biomaterials and Cell Signalling, York, Birleşik Krallık, 16 - 19 Temmuz 2000, ss.98 identifier

  • Yayın Türü: Bildiri / Tam Metin Bildiri
  • Basıldığı Şehir: York
  • Basıldığı Ülke: Birleşik Krallık
  • Sayfa Sayıları: ss.98
  • Ankara Üniversitesi Adresli: Evet

Özet

The use of biodegradable poly (DL-lactic-co-glycolic acid) (PLGA) scaffold as adrenal chromaffin cells (ACC) substrate that can enhance the viability and metabolic activity of the cells was studied. Morphological evidence showed the prolonged viability of chromaffin cells on the scaffolds having pores of 250-400 μm. It was observed that the majority of the seeded cells were located within the scaffold. It was also observed that the partially and highly purified ACC exhibited higher levels of leu- and met-enkephalin on PLGA scaffold compared with their monolayer cultures.