Akademik Veri Yönetim Sistemi
Microchemical Journal, cilt.225, 2026 (SCI-Expanded, Scopus)
Deoxyguanosine is highly susceptible to oxidative damage, particularly by hydroxyl radicals, leading to the formation of 8-hydroxy-2′-deoxyguanosine. This stable and widely recognized biomarker serves as an indicator of oxidative DNA damage in biological fluids. In this study, 8-hydroxy-2′-deoxyguanosine was successfully detected in artificial saliva using differential pulse adsorptive stripping voltammetry with an unmodified edge-plane pyrolytic graphite electrode in phosphate buffer solution (pH 6.9). Under optimized conditions, including an accumulation time of 480 s at 0.3 V (stirring at 250 rpm), the method demonstrated a linear response over the concentration range of 2.82 × 10−8 − 7.06 × 10−7 M in artificial saliva with limits of detection and quantification of 3.39 × 10−9 M and 1.03 × 10−8 M, respectively. Notably, this approach eliminates the need for electrode surface modification, offering a rapid and cost-effective electrochemical strategy for the quantification of 8-hydroxy-2′-deoxyguanosine.